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Question

A/B testing subject line

  • January 22, 2026
  • 3 replies
  • 12 views

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I have a few cadences where I want to A/B test the subject line in the first email. I then have 3 additional emails but when I choose ‘thread’ it only gives the option for ‘A’ in the previous email and not B. What happens when I launch the cadence? Will everyone getting the additional emails get threaded to the A option only (even if they receive the B option for the first email)?

 

TIA!

3 replies

TinaMaerina
Lofter
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  • Lofter
  • January 23, 2026

Hi ​@CCZerbey, thank you for your question. Nice to meet you, I’m Tina, a Senior Customer Success Manager at Salesloft.

 

To answer your question, variant testing is truly random so if a person gets variant A on the first step, they are still just as likely to be added to either variant A, C, or C on their second step. The nice thing is that with a threaded email, it is a true reference to the subject line they sent, so even if it looks like it is only allowing a reference to the first variant in the first step, it will automatically thread to the subject line of whatever variant they initially received.

 

Hope that answers your question! Happy lofting!


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  • Author
  • Champion
  • January 23, 2026

Thanks Tina! To confirm - I have 2 variants in email #1 (subject line 1 and subject line 2). Email #2 has only 1 variant BUT if someone received Email #1 subject line 1 they will thread with that subject line and if someone received email #1 subject line 2, they will thread with that subject line, right?


TinaMaerina
Lofter
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  • Lofter
  • January 23, 2026

@CCZerbey yes that’s correct 😊